Optimization of Zeesan SARS-COV-2 Test Kit for Porcine DNA Detection Tests on Processed Meat Products and Complex Processed Product

Various brands of covid 19 RNA detection kits are widely used by testing laboratories. One these is the Zeesan SARS COV-2 Test Kit. The kit cannot be to detect porcine DNA. There has been no official research on use optimized for DNA detection, thus optimization Kit a new innovation in molecular testing. purpose this study was find right combination between and primer-probe. Then look appropriate temperature setting amplification process Analysis using Liferiver brand real-time PCR with hydrolysis probe method. conducted running 3 types positive samples different concentrations. sample sausage, ham, noodles. results showed that Kit-primary successfully detected concentration 10 ng 5 ng. Meanwhile, at 1 ng, it could only 2 samples. In addition, relatively faster time than several previous studies.